Question 1
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).

Question 3
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

Question 4
What would be the molar concentration of human DNA in a human cell? Consult your teacher.

Question 5
Do eukaryotic cells have restriction endonucleases? Justify your answer.

Question 6
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?

Question 8
Can you recall meiosis and indicate at what stage a recombinant DNA is made?

Question 9
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?

Question 10
Describe briefly the followings:
(a) Origin of replication
(b) Bioreactors
(c) Downstream processing

Question 11
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase

Question 12
Discuss with your teacher and find out how to distinguish between
(a) Plasmid DNA and chromosomal DNA
(b) RNA and DNA
(c) Exonuclease and Endonuclease

Question 1
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because −
(a) bacteria are resistant to the toxin
(b) toxin is immature:
(c) toxin is inactive:
(d) bacteria encloses toxin in a special sac.

Question 2
What are transgenic bacteria? Illustrate using any one example.

Question 4
What are Cry proteins? Name an organism that produces it. How has man exploited this protein to his benefit?

Question 5
What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.

Question 6
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli ?

Question 7
Can you suggest a method to remove oil (hydrocarbon) from seeds based on your understanding of rDNA technology and chemistry of oil?

Question 9
Does our blood have proteases and nucleases?

Question 10
Consult internet and find out how to make orally active protein pharmaceutical. What is the major problem to be encountered?